Processing DNA samples can be a time consuming and expensive process. If degradation occurs during any of the steps, the process must start over.
Before you begin, make sure that the DNA is free of RNA and protein contamination. To prevent contamination of reagents by nucleases, always wear powder-free laboratory gloves and use dedicated solutions and pipettors with nuclease-free aerosol-resistant tips. Avoid repeated freeze-thaw cycles of solutions containing DNA or enzymes. Follow the procedure described in this document to isolate DNA from blood, cells, or frozen tissues, to increase the likelihood of a successful experiment.
General steps in DNA processing include:
1. Remove lipids by adding detergent
2. Removing proteins
3. Precipitate DNA with alcohol
4. Evaporating alcohol from supernatant using Labconco’s CentriVap micro IR
5. Storing the sample
If you have any questions, please call our Product Manager, Jenny Sprung, at (800) 821-5525 or email her at firstname.lastname@example.org. Also, visit our website and look under Support Documents on our CentriVap web pages for other application notes and a features and benefits video.
Purification of DNA, Open/Wetware- Openwetware.org/wiki/Purification_of_DNA (accessed in May, 2011) Microbial Life Educational Resources, George Rice, Montana State University- Serc.carleton.edu/microbelife/research_methods/genomics/dnaext.html University of Oklahoma's Advanced Center for Genome Technology, protocol part III. html Genome.ou.edu/protocol_book/protocol_partIII.html
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